Journal: eLife
Article Title: Single-cell profiling reveals the intratumor heterogeneity and immunosuppressive microenvironment in cervical adenocarcinoma
doi: 10.7554/eLife.97335
Figure Lengend Snippet: ( A ) Circle plots showing the interacting networks between epithelial cell sub-clusters and T cell sub-clusters via the pathway of ALCAM by comparing adenocarcinoma (ADC) and squamous cell carcinoma (SCC). ( B ) Circle plots simplified from ( A ) to show the interactions among target cell clusters via ALCAM pathway. ( C ) Circle plots showing the interacting networks between epithelial cell sub-clusters and T cell sub-clusters via the pathway of MHC-II by comparing ADC and SCC. ( D ) Circle plots simplified from ( C ) to show the interactions among target cell clusters via MHC-II pathway. From ( A ) to ( D ), the direction of each arrow shows the regulation from outputting cells to incoming cells. The width of the each line shows the predicted weight and strength of regulation. ( E ) Bubble plot showing the probability of ligand-to-receptor combination of each pathway between two different target sub-clusters of cells by comparing ADC with SCC. ( F ) Circle plots showing Tregs regulate epithelial cells via the TGF-β pathway, which is solely activated in ADC. ( G ) Bubble plot showing the probability of ligand-to-receptor combination of TGF-β pathway between Tregs and epithelial cells by comparing ADC with SCC. The pathways of ADGRE5, CD46, GZMA, and NAMPT are used as negative controls. ( H ) Dual immunofluorescence (IF) staining confirming that in SLC26A3 high regions of CC tissues, more FOXP3 + cells are recruited than in SLC26A3 low regions (left). The numbers of recruited FOXP3 + cell are quantified using histogram plot (right). Three individual samples with ROI were calculated and p<0.01 were marked with **, showing significant difference. ( I ) Multiplexed IF staining confirming the interaction between CD6 (on FOXP3 + cells) and ALCAM (on SLC26A3 high epithelial cells) in the ALCAM pathway. ( J ) Multiplexed IF staining showing that the recruitment of FOXP3 + cells toward SLC26A3 high cells might induce EMT (marked with E-cadherin) and increase the stemness (marked with ALDH1A1) of tumor cells, via TGF-β pathway.
Article Snippet: Antibody , ALCAM polyclonal antibody , Proteintech , Cat# 21972-1-AP , .
Techniques: Immunofluorescence, Staining
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